Propolis as a Potential Novel Histological Tissue Fixative: A Preliminary Analysis

Background: Fixation of biopsy specimens is a critical step before processing and staining them for histological tissue examination. Formalin considered the gold standard fixative solution its attributes. However, it has concerning side effects, such as carcinogenic potential irritational properties. Owing to various harmful safer natural substitute should be explored. In this study, we compared effectiveness propolis 10% formalin determined ability solution. Materials Methods: Sixty were collected from goats’ tongues immediately placed in (1) 6.6% propolis, (2) buffered (positive control), (3) followed by formalin, (4) 0.9% saline (negative control). Tissue samples fixed at different time points (12, 24, 48, 72 h) room temperature, staining. The quality microscopic parameters was blindly assessed two oral maxillofacial pathologists using numerical scoring scale. Scores statistically analyzed. Results: fixation significantly better than that points. Conclusion: Propolis showed promising properties can alternative formalin.